论文部分内容阅读
构建了含有pgk启动子驱动的HSV-tk基因的反转录病毒载体pLNTK，PCR证实pLNTK成功地转移至SHG44及NBA2细胞。体外实验证实，表达HSV-tk次的细胞对ACV的敏感性明显高于未修饰的亲本细胞，SHGLNTK、NBALNTK细胞对ACV的敏感性分别是其亲本细胞的1000、500倍。3H－TdR掺入法证实转染HSV－tk细胞在ACV存在下，其DNA合成能力较亲本细胞明显受抑制。共培养实验证实存在旁观者效应。
A retroviral vector pLNTK containing the pgk promoter-driven HSV-tk gene was constructed and confirmed by PCR that pLNTK was successfully transferred to SHG44 and NBA2 cells. In vitro experiments confirmed that cells expressing HSV-tk were more sensitive to ACV than unmodified parental cells, and SHGLNTK and NBALNTK cells were 1000 and 500 times more sensitive to ACV than their parental cells respectively. 3H-TdR incorporation confirmed that HSV-tk transfected HSV-tk cells in the presence of ACV, DNA synthesis capacity than the parental cells was significantly inhibited. Co-culture experiments confirmed the bystander effect.