目的观察内毒素和肿瘤坏死因子（ＴＮＦ）联合致休克模型大鼠胃肠运动功能改变及粘膜损伤的程度．方法采用Ｗｉｓｔａｒ♂大鼠，用内毒素和人重组ＴＮＦα联合致休克模型；将电极埋置在胃、十二指肠、空肠的浆膜面，记录大鼠的胃肠电活动，给大鼠灌胃碳末，测量碳末的推进速度，计数大鼠胃肠电快波频率（Ｆ）、振幅指数（ＡＩ）及碳末推进速度；取制膜后２ｈ的肠系膜淋巴结、心、肝、脾、肺、肾和血进行细菌培养，观察肠道细菌的移位情况；制膜１ｈ后给大鼠灌胃乳果糖／甘露醇混悬液，收集大鼠６ｈ尿液，用高压液相技术测定尿中乳果糖和甘露醇含量，计算它们排出率之比，测定肠粘膜的通透性．用χ２检验比较各组器官细菌感染率的不同，用ｔ检验统计各组间其他各指标的差异，Ｐ＜００５有显著性差异．结果①制模大鼠胃肠运动的改变：模型大鼠胃肠电活动明显抑制，快波的频率［Ｆｒｅ：胃电（１３２±０３０），十二指肠电（４４７±０７６），空肠电（４０７±１２５）次／ｍｉｎ］及振幅指数［ＡＩ：胃电（１３７０±３２３），十二指肠电（４３０６±８００），空肠电（３０７９±７４９）μＶ／ｍｉｎ］明显低于制模前［Ｆｒｅ：胃电（２９１±０１７），十二指肠电? Objective To observe the changes of gastrointestinal motor function and the degree of mucosal injury induced by endotoxin combined with tumor necrosis factor (TNF) in shock-induced shock rats. Methods Wistar male rats were used to induce the shock model with endotoxin and human recombinant TNFα. The electrodes were embedded in the serosal surface of the stomach, duodenum and jejunum and the gastrointestinal electrical activity of the rats was recorded. Rats were sacrificed and the carbon powder was instilled into the stomach to measure the advancing speed of carbon powder. The frequency of gastrointestinal fast wave (F), amplitude index (AI) and carbon advancing velocity were counted. Mesenteric lymph nodes, heart, liver, Spleen, lung, kidney and blood were cultured in order to observe the translocation of intestinal bacteria. After 1 h of membrane preparation, lactulose / mannitol suspension was intragastrically administered to rats and the urine of rats was collected for 6 h. High pressure liquid phase Determination of urinary lactulose and mannitol content, calculate the ratio of their discharge rate, the determination of intestinal mucosal permeability. Χ2 test was used to compare the bacterial infection rates of different organs. The t-test was used to calculate the difference of other indexes in each group, P <005 was significantly different. Results ① The change of gastrointestinal motility in model rats: The gastrointestinal electrical activity was significantly inhibited in model rats, the frequency of fast wave [Fre: Gastroscopy (1.32 ± 0.30), Duodenal (4.47 ± 076), jejunum (40 ± 125) times / min and amplitude index [AI: electrocardiogram (1370 ± 323), duodenal electricity (4306 ± 8) 00), jejunum (3079 ± 749) μV / min] was significantly lower than that before modeling [Fre: electrogastrogram (291 ± 017), duodenal electro-