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目的 了解狼疮肾炎 (LN)病人白细胞介素 17(IL 17)在血浆、尿液及肾组织中表达水平与LN发病相关性。方法 建立正常对照、活动期和非活动期LN组 ,用酶联免疫吸附法(ELISA)测定其血浆和尿液IL 17水平 ;制备正常对照、WHOⅣ型和非Ⅳ型肾组织标本 ,采用微波免疫组织化学染色法 ,光镜观察IL 17在肾组织中的表达。结果 血浆IL 17均测不出 (<5 pg/ml) ;尿液IL 17水平 ,活动期LN病人 (2 9± 5 ) pg/ml较正常对照 (3 3± 0 8)pg/ml和非活动期LN病人(9 6± 0 9) pg/ml明显升高 (P <0 0 0 1) ,且非活动期LN病人较正常对照亦明显升高 (P <0 0 0 1) ;免疫组织化学显示 ,WHOⅣ型LN较非Ⅳ型和正常对照组 ,单位肾小球细胞数 [(117± 31) / gcs(肾小球横向联合切面 )较 (4 3± 8) /gcs和 (34± 7) / gcs]明显升高 (P <0 0 1) ,单位肾小球IL 17+ 细胞数(Ng) [(33± 8) /gcs较 (8 2± 1 6 ) / gcs和 0 ]明显增多 (P <0 0 1) ,每平方毫米肾小管、间质IL 17+ 细胞数 (Nt) [(2 9± 9) /mm2 较 (8 8± 1 5 ) /mm2 和 0 ]亦明显增多 (P <0 0 1)。相关分析显示Ng与肾小球细胞增多、肾组织活动指数、尿蛋白、尿N 乙酰 β 氨基葡萄糖苷酶 (NAG)、血肌酐及尿IL 17亦呈正相关 ;Nt与肾组织活动指数、肾小管间质损害程度、尿?
Objective To investigate the correlation between the expression of interleukin-17 (IL-17) and LN in plasma, urine and kidney of patients with lupus nephritis (LN). Methods The normal control, active and inactive LN groups were established. The levels of IL-17 in plasma and urine were determined by enzyme-linked immunosorbent assay (ELISA). The normal control, WHOⅣtype and non-Ⅳtype renal tissue samples were prepared and immunized by microwave Histochemical staining and light microscopy were used to observe the expression of IL-17 in renal tissue. Results Plasma levels of IL-17 were not detected (<5 pg / ml); urinary IL-17 levels were significantly higher in active LN patients (29 ± 5) pg / ml than in normal controls Active LN patients (96 ± 0 9) pg / ml were significantly higher (P <0.01), and inactive LN patients were also significantly higher than the normal control (P <0.01); immune tissue The results showed that the number of glomerular cells per unit glomerular cell number [(117 ± 31) / gcs (43 ± 8) / gcs and (34 ± 7) / gcs] (P <0.01), and the number of glomerular IL 17+ cells (Ng) [(33 ± 8) / gcs vs (82 ± 1 6) / gcs and 0] (P <0.01), and the number of IL 17+ cells per square millimeter (tubal interstitial tubules, interstitial tubules and mesangial cells) also increased significantly (29 ± 9 / mm 2 vs 8 8 ± 1 5 / mm 2 and 0] (P <0 0 1). Correlation analysis showed that Ng was positively correlated with increased glomerulus, renal activity index, urinary protein, NAG, serum creatinine and urinary IL-17. Nt was positively correlated with renal tissue activity index, The degree of interstitial damage, urine?