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以萝卜雄性不育系8418A及其保持系8418B为材料,经不连续Percol梯度分离出完整叶绿体,以35S-甲硫氨酸标记离体叶绿体合成的蛋白质,对类囊体膜蛋白和基质蛋白分别经SDS-PAGE分离和放射自显影,发现由叶绿体基因组编码的类囊体膜蛋白和基质蛋白在不育系和保持系间有明显差异。
The intact chloroplast was isolated from the male sterile line 8418A of radish and its maintainer line 8418B by discontinuous Percol gradient. The protein synthesized from the detached chloroplast was labeled with 35S-methionine, and the membrane protein and matrix protein of the thylakoid After SDS-PAGE separation and autoradiography, it was found that the thylakoid membrane protein and matrix protein encoded by the chloroplast genome were significantly different between male sterile line and maintainer line.