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50年代初,Sutherland就注意到肾上腺素促进糖元分解是通过细胞内的一种介质物质。尔后发现这种物质就是环-磷酸腺苷(cAMP),它不仅与糖元代谢有关,而且是生命现象的重要调节物质。先前报告,热暴露引起家兔体温升高后,血浆中环核苷酸含量显著升高,体温与cAMP、cGMP含量呈正相关。为探讨血浆中cAMP含量与体温调节的关系,本研究观察了猕猴热应激和热服时血浆中cAMP的变化 实验方法 健康成年猕猴8只,于热箱内(Td41℃、Tw38℃)热暴露至肛温达40.4±0.2℃,记录时间,每天一次,连续10天。热习服期前后,于同样的热环境下进行标准高温试验至肛温达41.6℃,紧接标准试验前后用蛋白竞争结合法测定血浆中cAMP含量。实验结果以M±Sk表示,并进行t测验。
In the early 1950s, Sutherland noticed that adrenergic glycogenolysis is a substance that passes through the cells. It was later discovered that this substance is cyclic-adenosine monophosphate (cAMP), which is not only related to glycogen metabolism, but also an important regulator of life phenomena. Previous reports, heat exposure caused rabbit body temperature, plasma cyclic nucleotide content was significantly increased, body temperature and cAMP, cGMP content was positively correlated. In order to investigate the relationship between cAMP content in plasma and thermoregulation, we observed the changes of cAMP in plasma after heat stress and warm-up in experimental macaques. Eight adult healthy rhesus monkeys were exposed to heat in a hot box (Td41 ℃, Tw38 ℃) Rectal temperature reached 40.4 ± 0.2 ℃, recording time, once a day for 10 days. Before and after the hot acclimatization period, the standard high temperature test was conducted in the same hot environment until the rectal temperature reached 41.6 ° C. The plasma cAMP content was measured by the protein competition binding method immediately before and after the standard test. The experimental results are expressed as M ± Sk and the t test is performed.