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对硬组织进行酶活性研究,最大的问题是难于制作切片。用一般酸性脱钙液脱钙,首先必须固定,否则会出现组织肿胀,结构破坏。而固定剂和酸性脱钙液对酶活性均有严重影响。60年代初,Balogh 用中性10%乙二胺四乙酸钠(EDTA)脱钙液(0.1M 磷酸缓冲液,pH7.0配制)作硬组织脱钙,发现其可保存一些脱氢酶的活性。随后,Fullmer 等(1964)系统研究了不同缓冲液配制 EDTA 脱钙液的脱钙效果及脱钙时间对酶活性的影响。他们提出,用三羟甲基氨基甲烷(tris)缓冲液的
On the hard tissue enzyme activity research, the biggest problem is difficult to make slices. Decalcification with the general acidic decalcification, the first must be fixed, otherwise there will be tissue swelling, structural damage. The fixative and acidic decalcification have a serious impact on enzyme activity. In the early 1960s, Balogh was hard-decalcified with a decalcification solution of neutral 10% EDTA (0.1 M phosphate buffer, pH 7.0) and found to retain some dehydrogenase activity . Subsequently, Fullmer et al. (1964) systematically studied the decalcification effect of EDTA decalcification solution prepared by different buffers and the effect of decalcification time on enzyme activity. They are presented with tris buffer