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用荧光光谱法研究了柠檬酸钠与牛血清白蛋白(BSA)的作用机制。实验表明,柠檬酸钠对BSA具有荧光猝灭作用,其猝灭方式为静态猝灭,并求出了猝灭常数、结合常数及结合位点数。
The mechanism of action of sodium citrate and bovine serum albumin (BSA) was studied by fluorescence spectroscopy. Experiments show that sodium citrate has a fluorescence quenching effect on BSA, and its quenching mode is static quenching, and the quenching constants, binding constants and the number of binding sites are obtained.