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目的：探讨哮喘时嗜酸性粒细胞（Ｅｏｓ）浸润与ＩＬ－５ｍＲＮＡ表达的关系以及糖皮质激素对其影响。方法：采用孵蛋白（ＯＡ）吸入致敏制作豚鼠哮喘模型，在诱发哮喘２４ｈ后，检测支气管肺泡灌洗液（ＢＡＬＦ）中不同密度的Ｅｏｓ的数量及其百分比，肺组织中原位ＩＬ－５ｍＲＮＡ的表达，以及地塞米松预防用药６ｄ对其变化的影响。结果：①哮喘组ＢＡＬＦ中Ｅｏｓ总数增加，低密度Ｅｏｓ（ＨＥｏ）增加尤为显著；肺组织中ＩＬ－５ｍＲＮＡ原位表达水平显著增加（Ｐ＜０．０１）；②与哮喘组比，地塞米松预防用药后Ｅｏｓ计数及ＨＥｏ百分比显著减少（Ｐ＜０．０１）；③相关分析发现，ＩＬ－５ｍＲＮＡ表达水平与Ｅｏｓ总数（ｒ＝０．６８８，Ｐ＜０．０１）及ＨＥｏ百分比显著正相关（ｒ＝０．５８２，，Ｐ＜０．０１）。结论：哮喘豚鼠肺组织ＩＬ－５ｍＲＮＡ表达的增加与ＢＡＬＦ中Ｅｏｓ数量增多密切相关。抑制ＩＬ－５ｍＲＮＡ表达可能是糖皮质激素减少Ｅｏｓ浸润及治疗支气管哮喘的重要机制之一。
Objective: To investigate the relationship between eosinophil (Eos) infiltration and IL-5 mRNA expression in asthma and the effect of glucocorticoids on it. Methods: The guinea pig asthma model was induced by the inhalation of hatched protein (OA). The numbers and percentages of Eos at different densities in bronchoalveolar lavage fluid (BALF) were detected 24h after induction of asthma. The levels of IL-5 mRNA Expression, and dexamethasone prophylaxis 6d on the changes. Results: (1) The total number of Eos in BALF and the low density Eos (HEo) increased significantly in asthmatic group. The expression of IL-5 mRNA in lung tissue was significantly increased (P <0.01). Compared with asthma group, dexamethasone (P <0.01); ③The correlation analysis showed that the level of IL-5 mRNA was significantly and positively correlated with the total number of Eos (r = 0.688, P <0.01) and HEO percentage (R = 0.582, P <0.01). Conclusion: The increased expression of IL-5mRNA in lung tissue of asthmatic guinea pigs is closely related to the increase of Eos in BALF. Inhibition of IL-5 mRNA expression may be one of the important mechanisms of glucocorticoid in reducing Eos infiltration and treating bronchial asthma.