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从微小体积鼻咽癌活检标本中获取纯净癌细胞一直是鼻咽癌分子生物学研究中的难题.为了寻找一种能从鼻咽癌活检组织中获得高纯度、高质量RNA来完成cDNA微阵列(cDNA Microarray)实验的简便实用方法,采用RNAlater技术保存鼻咽癌活检组织,显微切割技术来获得高纯度鼻咽癌细胞,利用RNA线性扩增技术得到cDNA微阵列实验所需RNA.结果表明:利用RNAlater技术可以很好地保持组织RNA的稳定,通过优化显微切割和RNA线性扩增的条件获得了cDNA微阵列实验所需的高纯度、高质量RNA.
Obtaining pure cancer cells from small volume nasopharyngeal carcinoma biopsy specimens has been a difficult problem in molecular biology research of nasopharyngeal carcinoma.In order to find a way to obtain high purity and high quality RNA from nasopharyngeal carcinoma biopsy tissue to complete cDNA microarray (RNA Microarray) experiment, RNAlater technology was used to preserve nasopharyngeal carcinoma biopsy tissue, and microdissection technique was used to obtain high-purity nasopharyngeal carcinoma cells, and the RNA required for cDNA microarray experiment was obtained by RNA linear amplification.The results showed that : Using RNAlater technology to maintain the stability of tissue RNA, high-purity, high-quality RNA required for cDNA microarray experiments was obtained by optimizing the conditions of microdissection and RNA linear amplification.