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AIM: The finding that some Helicobacterpyloristrains expressLewis b (Leb) blood group antigen casts a doubt on the roleof Leb of human gastric epithelium being a receptor for-H.pylori. The aim of this study was to determine if expressionof Leb in H. Pyloriinterferes with bacterial adhesion property.METHODS: Bacterial adhesion to immobilized Leb onmicrotitre plate was performed in 63-H. Pyloristrains obtainedfrom Singapore using in vitro adherence assay. Expression ofLewis blood group antigens was determined by ELISA assay.RESULTS: Among 63 H. Pyloristrains, 28 expressed Lebantigen. In vitro adhesion assay showed that 78.6 % (22/28) of Leb-positive and 74.3 % (26/35) of Leb-negative-H.pyloriisolates were positive for adhesion to immobilized Lebcoated on microtitre plate (P＝0.772). In addition, blockingof H. Pylori Leb by prior incubation with anti-Leb monoclonalantibody did not alter thebinding of the bacteria to solid-phase coated Leb.CONCLUSION: The present study suggests that expressionof Leb in H. Pyloridoes not interfere with the bacterialadhesion property. This result supports the notion that Lebpresent on human gastric epithelial cells is capable of beinga receptor for H.pylori.