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目的探讨昆明地区汉族人群血管紧张素原(AGT)基因及血管紧张素转换酶(ACE)基因多态性与糖尿病肾病(DN)的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测T2DM不伴DN患者74例(T2DM组)、DN患者120例(DN组)及60名健康体检者(对照组)的ACE I/D及AGT M235T基因型。结果 (1)DN组ACE-DD基因型频率和D等位基因频率(分别为53%、71%)均高于T2DM组(分别为22%、46.6%)及对照组(分别为29%、45.5%)(P均<0.01)。(2)AGT M235T基因型频率和等位基因频率三组差异无统计学意义(P>0.05)。(3)同时携带ACE-DD及AGT-TT基因型者发生DN的OR值为6.183。结论 (1)ACE-DD基因型和D等位基因可能是昆明地区汉族人群DN的易感因素。(2)未发现AGT M235T多态性单一因素与DN的发生有关联。(3)ACE-DD基因型与AGT-TT基因型可能在DN发生中有协同作用。
Objective To investigate the relationship between angiotensinogen (AGT) gene and angiotensin converting enzyme (ACE) gene polymorphism and diabetic nephropathy (DN) in Han population in Kunming area. Methods Totally 74 patients with T2DM without DN (T2DM group), 120 with DN (DN group) and 60 healthy subjects (control group) were enrolled in this study by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) ) ACE I / D and AGT M235T genotypes. Results The frequencies of ACE-DD genotypes and D allele in DN group (53%, 71%, respectively) were significantly higher than those in T2DM group (22% vs. 46.6%, respectively) and controls (29% 45.5%) (all P <0.01). (2) There was no significant difference in AGT M235T genotype frequency and allele frequency between the three groups (P> 0.05). (3) The odds ratio (DN) of patients with both ACE-DD and AGT-TT genotypes was 6.183. Conclusions (1) ACE-DD genotype and D allele may be the predisposing factors of DN in Kunming Han population. (2) A single factor of AGT M235T polymorphism was not associated with the occurrence of DN. (3) ACE-DD genotype and AGT-TT genotype may have a synergistic effect in the pathogenesis of DN.