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目的通过观察消风Ⅱ号胶囊对佐剂性关节炎(AA)大鼠炎性组织中前列腺素E2(PGE2)及一氧化氮(NO)含量的影响,探讨其治疗类风湿关节炎作用机理。方法将AA大鼠随机分为模型组及消风Ⅱ号胶囊低(188 mg/kg)、高剂量组(375 mg/kg),同时设正常组。致炎第7日开始灌胃给予药物,灌胃剂量为1.0 mL/100 g体质量,模型组给予等体积的蒸馏水,连续21 d。给药结束后次日处死大鼠,测量AA大鼠炎性组织中PGE2及NO含量。结果消风Ⅱ号胶囊低、高剂量组均可抑制大鼠非佐剂注射侧炎性组织中PGE2的生物合成,抑制率分别为41.2%和33.6%,抑制大鼠佐剂注射侧炎性组织中NO含量,抑制率分别为39.9%和29.7%,与模型组相比,差异有统计学意义(P<0.001,P<0.01)。结论消风Ⅱ号胶囊抗关节炎作用通过抑制PGE2和NO的合成或释放,使局部炎性组织中PGE2及NO含量下降而发挥作用。
Objective To observe the effect of Xiaofeng Ⅱ capsule on the content of prostaglandin E2 (PGE2) and nitric oxide (NO) in the inflammatory tissues of rats with adjuvant arthritis (AA), and to explore its mechanism of action in the treatment of rheumatoid arthritis. Methods AA rats were randomly divided into model group and Xiaofeng Ⅱ capsule (188 mg / kg), high dose group (375 mg / kg), and normal group. On the 7th day, the rats were given gavage with a dose of 1.0 mL / 100 g body weight. The model group was given equal volume of distilled water for 21 days. The rats were sacrificed the next day after the administration, and the contents of PGE2 and NO in the inflammatory tissues of AA rats were measured. Results Xiaofeng Ⅱ capsule could inhibit the biosynthesis of PGE2 in the non-adjuvant injected side inflammatory tissues with the inhibition rates of 41.2% and 33.6% The content of NO and the inhibition rate were 39.9% and 29.7%, respectively. Compared with the model group, the difference was statistically significant (P <0.001, P <0.01). Conclusion Xiaofeng Ⅱ capsule has anti-arthritic effect by inhibiting the synthesis or release of PGE2 and NO and decreasing the content of PGE2 and NO in local inflammatory tissues.