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目的筛选放线菌来源的棘白霉素B脱酰基酶产生菌,并对其转化条件进行优化。方法根据菌株转化产物对白念珠菌的抑制活性进行初筛,再以HPLC、LC-MS检测确定目的菌株;对目的菌株转化棘白霉素B的培养基、转化温度和时间、缓冲液、甲醇含量等条件进行优化以提高转化率。结果筛选得到4株可产生脱酰基酶的阳性菌株,其中,N07W-.61为小单孢菌属菌株,其余3株为链霉菌属菌株;通过转化条件优化,菌株N07W-61对棘白霉素B的转化率从2.60%提高至61.24%。结论小单孢菌属菌株产生棘白霉素B脱酰基酶为首次报道;该属菌株N07W-61在优化转化条件下对棘白霉素B的转化率有了大幅提高。
Objective To screen actinomycete-derived deacylase from actinomycetes and optimize its transformation conditions. Methods The inhibitory activity of Candida albicans was determined according to the transformation products of the strain. The target strain was determined by HPLC and LC-MS. The target strain was transformed into the culture medium of Spinacin B, the temperature and time of transformation, the buffer and the content of methanol And other conditions to optimize the conversion rate. Results Four strains were screened to produce deacylase. Among them, N07W-.61 was a member of the genus Micromonospora and the other three strains were Streptomyces strains. Through optimization of the transformation conditions, strain N07W-61 The conversion of element B increased from 2.60% to 61.24%. Conclusions It is the first report of the production of acteomycin B deacylase from Micromonospora strains. The strain N07W-61 has a significant increase in the conversion of echinocandin B under optimal conditions.