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目的探讨脑钠肽(BNP)在实验性大鼠急性心肌梗死(AMI)早期4 h内的变化。方法25只Wistar大鼠制作AMI模型,20只大鼠行假手术作为对照组,两组实验前后作心肌电镜观察。采用双抗体夹心酶联免疫吸附试验(ELISA)法检测实验前后1~4 h大鼠血清BNP和心肌肌钙蛋白I(cTnI)浓度。结果成功建立AMI模型后1~4 h,大鼠血清BNP浓度较实验前和对照组显著升高(P均<0.01),以实验后2h大鼠血清BNP浓度最高(520.66±16.85)ng/L;AMI组大鼠1~4 h血清cTnI浓度较实验前及对照组差异无统计学意义(P均>0.05)。自建立AMI模型后1~4 h,大鼠血清BNP浓度与AMI范围呈显著正相关,以2 h大鼠血清BNP浓度与AMI范围相关性最高(r=0.69,P<0.05)。对照组实验前后血清BNP和cTnI浓度差异无统计学意义(P均>0.05)。结论BNP可以作为提示早期AMI并可以同时预测梗死区大小的生化标志物。
Objective To investigate the changes of brain natriuretic peptide (BNP) in the first 4 hours of experimental acute myocardial infarction (AMI) in rats. Methods Twenty-five Wistar rats were used to make the AMI model. Twenty rats were sham-operated as the control group. The two groups were observed before and after the myocardial electrocardiography. Serum levels of BNP and cardiac troponin I (cTnI) were measured by ELISA before and after 1 ~ 4 h. Results The levels of serum BNP were significantly increased at 1 ~ 4 h after AMI model establishment (P <0.01), and the highest BNP level was 520.66 ± 16.85 ng / L The serum concentration of cTnI in AMI rats after 1 ~ 4 h was not significantly different from that before the control and in the control group (all P> 0.05). Serum BNP concentration was positively correlated with AMI range 1 ~ 4 h after establishment of AMI model, and serum BNP concentration had the highest correlation with AMI range at 2 h (r = 0.69, P <0.05). There was no significant difference in serum BNP and cTnI concentrations before and after the control group (all P> 0.05). Conclusions BNP can be used as a biochemical marker to predict early AMI and predict infarct size at the same time.