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目的探讨上皮-钙黏素(E-cadherin)、神经-钙黏素(N-cadherin)及β-链蛋白(β-catenin)在精原细胞瘤中的表达及意义。方法采用S-P免疫组化法检测29例精原细胞瘤及10例正常睾丸E-cadherin、N-cadherin、β-catenin的表达水平,并进一步通过RT-PCR及蛋白免疫印迹技术(5例精原细胞瘤、5例正常睾丸)验证。结果免疫组化染色结果显示,正常睾丸细胞间连接处可见N-cadherin表达,而精原细胞瘤中未见N-cadherin表达;精原细胞瘤E-cadherin、β-catenin表达水平较正常睾丸显著下降,差异有显著性(P<0.05);精原细胞瘤E-cadherin、β-catenin表达水平与肿瘤TNM分期相关性分析结果无显著性(P>0.05,r1=0.2669,r2=-0.2280)。免疫印迹及PCR结果显示,精原细胞瘤E-cadherin、N-cadherin、β-catenin表达水平较正常睾丸显著下降,差异有显著性(P<0.05)。结论精原细胞瘤中E-cadherin-β-catenin复合体及N-cadherin表达较正常睾丸显著下降,且与肿瘤TNM分期无显著相关,提示E-cadherin、β-catenin及N-cadherin的降低可能参与精原细胞瘤的癌变过程,但与肿瘤侵袭及转移过程无显著相关。
Objective To investigate the expression of E-cadherin, N-cadherin and β-catenin in seminoma and its significance. Methods The expression of E-cadherin, N-cadherin and β-catenin in 29 cases of seminoma and 10 cases of normal testes were detected by SP immunohistochemical method. The expression of E-cadherin and β-catenin were detected by RT-PCR and Western blotting Cell tumor, 5 normal testes). Results The results of immunohistochemistry showed that N-cadherin expression was found in normal testicular cell junctions, but not in seminoma. The expression of E-cadherin and β-catenin in seminoma was significantly higher than that in normal testis (P <0.05). There was no significant correlation between the expression of E-cadherin and β-catenin in seminoma and TNM staging (P> 0.05, r1 = 0.2669, r2 = -0.2280) . Western blotting and PCR showed that the expression of E-cadherin, N-cadherin, and β-catenin in seminoma was significantly lower than that in normal testes (P <0.05). Conclusions The expression of E-cadherin-β-catenin complex and N-cadherin in seminoma is significantly lower than that in normal testes and not significantly correlated with the TNM stage of the tumor, suggesting that the decrease of E-cadherin, β-catenin and N-cadherin may be Participate in the carcinogenesis of seminoma, but have no significant correlation with tumor invasion and metastasis.