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目的 :包虫病是一种危害性很大的人畜共患病 ,除了甲苯咪唑 (MBZ)和阿苯达唑 (ABZ)这两种公认的有效药物以外 ,目前尚无新的更有效的药物出现。本实验通过体外试验进行了氧苯达唑 (OBZ)、阿苯达唑和阿苯达唑脂质体 (L - ABZ)的体外抗细粒棘球蚴原头节作用的比较 ,探讨氧苯达唑在抗包虫病方面的作用 ,为探索新的抗包虫病药物奠定基础。方法 :将氧苯达唑、阿苯达唑和阿苯达唑脂质体分别加入 RPMI16 40培养液中 ,配成高 (2 5 0 0 ng/ml)、中 (5 0 0 ng/ ml)和低 (10 0 ng/ ml) 3个浓度的加药培养液 ,将用无菌方法采集的自然感染的羊细粒棘球蚴原头节 (活头节占 95 %以上 )加入上述加药培养基中进行培养 ,每天计数其死亡率 ,方法如下 :取一滴培养液 ,0 .1%伊红溶液染色 5 min,在普通光镜下观察 ,死亡原头节红染着色 ,伴有结构破坏 ;活头节则拒染不着色 ,计数每一视野内着色 /不着色头节数目 ,以着色者所占观察数之百分比作为原头节的死亡率 ,观察 3个视野 ,计算平均死亡率。每天计数 1次 ,直到对照组的原头节也全部死亡为止。实验过程中不更换培养基。结果 :(1)原头节死亡率氧苯达唑、阿苯达唑、阿苯达唑脂质体与对照组相比均有统计学意义 (P <0 .0 5 ) ,且氧苯达唑、阿苯达唑在低浓度时与对照组相比有统计?
OBJECTIVE: Echinococcosis is a potentially harmful zoonotic disease. There are no new and more effective drugs besides MBZ and ABZ appear. In this study, in vitro experiments against oxalindazole (OBZ), albendazole and albendazole liposomes (L - ABZ) in vitro against protoscolex Echinococcosis compared to explore the role of benzene The role of dazole in combating echinococcosis lay the foundation for exploring new anti-echinococcosis drugs. METHODS: Oxybenzone, albendazole and albendazole were added into RPMI1640 culture medium respectively to make them high (2500 ng / ml) and medium (500 ng / ml) And low (10 0 ng / ml) dosing culture medium were added to the above-mentioned dosing of naturally infected goat echinococcosis section (above 95% of the live heads) collected by aseptic method The culture was carried out and the mortality was calculated daily. The method is as follows: one drop of culture solution was taken, and the 0.1% Eosin solution was dyed for 5 min. The result was observed under a normal light microscope. ; Live head section is not stained irreducible, count the number of colored / uncolored head in each field of vision, the percentage of the number of shaders to observe the mortality of the original head section, observe the three fields of view, calculate the average mortality. Count 1 times a day, until the control group of the original head section also died. The medium was not changed during the experiment. Results: (1) Oxybutynol, albendazole, and albendazole were all significantly decreased in the mortality of protoscoleces compared with the control group (P <0.05) Azoles, albendazole at low concentrations compared with the control group statistics?