克隆了6个不同基因型大豆的Fd-GOGAT基因,序列比对分析结果表明:不同基因型大豆的Fd-GOGAT基因序列相似性很高,可设计出通用Real-time PCR引物,检测Fd-GOGAT基因的表达规律。在东农42 Fd-GOGAT基因序列的编码区内出现了1个8核苷酸的缺失,产生了移码突变,导致其C端缺失了79个氨基酸的保守序列,造成谷氨酸合成酶大亚基C端保守结构域(gltB_C)不完整。Fd-GOGAT基因分子进化树和蛋白质序列分子进化树都显示东农42、半野生大豆、东农46 Fd-GOGAT基因序列进化距离较近。 Fd-GOGAT gene was cloned from 6 different genotypes of soybean. Sequence alignment analysis showed that Fd-GOGAT gene sequences of different genotypes of soybean were highly similar. Universal Real-time PCR primers were designed to detect Fd-GOGAT Gene expression patterns. A deletion of 8 nucleotides in the coding region of the Fd-GOGAT gene sequence of Dongnong42 resulted in a frameshift mutation, resulting in a deletion of a conserved sequence of 79 amino acids at the C terminus, resulting in a large glutamase synthase Subunit C-terminal conserved domain (gltB_C) is incomplete. Fd-GOGAT gene phylogenetic tree and protein sequence molecular phylogenetic tree showed Dongnong 42, semi-wild soybean, Dongnong 46 Fd-GOGAT gene sequence evolution closer.