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One-day-old healthy ducklings (n=100) were divided into control group and experimental group randomly, and selenium poisoning of ducklings was artificially caused by feeding ration containing 8 lng kg-1 Se every day. The dynamic changes of No content and NOS activity in the serum and tissues were determined by means of the method of nitric acid reductase. Results showed that the NO content and NOS activity in serum and tissues in experimental group increased significantly (P＜0.05) and they were time-dependent.It was suggested that the high level of selenium in bodies could increase the NOS activity and NO content as it destroyed the metabolism of material and energy as well as structure and function of tissues and cells. These changes of NO content and NOS activity might be involved in the metabolism of selenium poisoning.