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目的:磷酸化蛋白激酶(Akt)是重要的促细胞存活信号,本研究观察双侧双极经颅直流电刺激(tDCS)模式对大鼠脑组织中Akt激活水平(即Akt的磷酸化水平)的影响。方法:将SD大鼠随机分成对照组和tDCS处理组,釆用不同电流参数不同时间刺激后,用免疫印迹法检测脑组织中Akt磷酸化水平(p-Akt)及Akt总蛋白量(tAkt)表达。计算p-Akt/t-Akt比值定量Akt磷酸化水平。结果:高强度刺激(250μA;2h)降低Akt磷酸化水平(P<0.05);低强度刺激(100μA;1h)明显上调Akt磷酸化水平,并持续上调12h(P<0.05)。结论:低强度双侧双极tDCS在12h内持续增强Akt活性,但过强tDCS引起Akt活性降低。
OBJECTIVE: Phosphorylation of protein kinase (Akt) is an important signal of cell survival. In this study, we observed the effect of bipolar transcranial direct current stimulation (tDCS) on the activation of Akt in rat brain (ie, the phosphorylation of Akt) influences. Methods: The SD rats were randomly divided into control group and tDCS treatment group. After stimulating with different current parameters for different time, the levels of Akt phosphorylation (Akt) and Akt total protein (tAkt) in brain tissue were detected by Western blot. expression. The level of Akt phosphorylation was quantified by calculating the ratio of p-Akt / t-Akt. Results: High-intensity stimulation (250μA; 2h) reduced Akt phosphorylation (P <0.05); low-intensity stimulation (100μA; 1h) significantly increased Akt phosphorylation and continued to increase 12h (P <0.05). CONCLUSION: Low-intensity bilateral bipolar tDCS can continuously enhance Akt activity within 12 hours, but excessive tDCS causes decreased Akt activity.