论文部分内容阅读
本研究利用ＣＤ３、ＣＤ４及ＣＤ８单克隆抗体对猪外周血淋巴细胞、胸腺细胞、扁桃体和肠系膜淋巴结淋巴细胞的组成进行了流式细胞仪分析。结果表明，胸腺中大胸腺细胞和小胸腺细胞的ＣＤ３、ＣＤ４及ＣＤ８的表达不同，在大胸腺细胞中， ＣＤ３的表达比小胸腺细胞的 ＣＤ３表达要高出 １０个百分点。小胸腺细胞中 ＣＤ４~＋ ＣＤ８~＋细胞约占 ６０％，而大胸腺细胞中只占 ３０％；相反，大胸腺细胞中 ＣＤ４~＋ ＣＤ８~＋细胞比小胸腺细胞要高。与外周血比较起来，肠系膜淋巴结和外周血有相似的淋巴细胞组成， ＣＤ４~＋和 ＣＤ８~＋细胞（包括 ＣＤ４~＋ ＣＤ８~＋细胞）占细胞总数 ６０％以上，而扁桃体中只占 ３０％， ＣＤ４~＋ＣＤ８~＋的比例相比较也要低。
In this study, the composition of porcine peripheral blood lymphocytes, thymocytes, tonsils and mesenteric lymph node lymphocytes were analyzed by flow cytometry using monoclonal antibodies against CD3, CD4 and CD8. The results showed that the expression of CD3, CD4 and CD8 in thymus thymus and thymus were different. In large thymocytes, the expression of CD3 was 10% higher than that of small thymocytes. Small thymocytes CD4 ~ + CD8 ~ + cells account for about 60%, while only 30% of large thymocytes; on the contrary, large thymocytes CD4 ~ + CD8 ~ + cells than small thymocytes. Compared with peripheral blood, mesenteric lymph nodes and peripheral blood have similar lymphocyte composition, CD4 ~ + and CD8 ~ + cells (including CD4 ~ + CD8 ~ + cells) accounted for more than 60% of the total cells, and tonsils only 30% , CD4 ~ + CD8 ~ + ratio is also lower.