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利用FsLinkageMap2.0和JoinMap4.0软件构建响叶杨×银白杨F1遗传整合图谱,在重组率为0.245时,二者构建的遗传框架图都包含20个主要的连锁群(4个以上标记),但2个遗传框架图的图距大小差异显著,利用JoinMap4.0构建的遗传图谱的标记排列顺序更加符合杨树一致性图谱上的标记排列顺序。估计杨树基因组总长度为2695.56cM,图谱的基因组覆盖度为93.34%。将利用MapMaker3.0和FsLinkageMap2.0构建的母本响叶杨的2个遗传图谱进行对比,共检测到32个对应连锁群和113个相同标记,利用FsLinkageMap2.0构建的响叶杨的遗传图谱上共显性标记的排列顺序与标记在杨树全基因组中的相对位置更符合。
The genetic linkage map was constructed using FsLinkageMap2.0 and JoinMap4.0 software. At the recombination rate of 0.245, the genetic frameworks constructed by the two included 20 major linkage groups (more than 4 markers) However, the size difference of the two genetic framework maps is significant. The order of the genetic maps constructed by using JoinMap 4.0 is more in line with the order of the markers on the poplar consensus map. The total length of the poplar genome is estimated to be 2695.56 cM, with a genome coverage of 93.34%. The genetic map of Populus simonii constructed by using MapMaker3.0 and FsLinkageMap2.0 was compared, and 32 corresponding linkage groups and 113 identical markers were detected. The genetic map of Populus simonii constructed by FsLinkageMap2.0 The arrangement of co-dominant markers is more in line with the relative position of markers in poplar genome.