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目的通过观察人乳腺癌组织中CDlc、S-100、HLA-DR阳性细胞数和表达强度的变化及其与T细胞的关系,了解乳腺癌局部微环境的免疫状态,探讨树突状细胞与乳腺癌发生、发展的关系,为乳腺癌的生物治疗提供实验依据。方法收集手术切除的人乳腺癌组织和癌旁相对正常乳腺组织,用免疫组织化学方法和图像分析技术对20例乳腺癌标本进行检测。结果乳腺癌组织中CDlc、S-100和HLA-DR的表达强度均较相对正常乳腺组织明显减弱(P<0.05);CD1c+DC、S-100+DC、HLA-DR+DC、CD4+T细胞和CD8+T细胞的数量均较相对正常乳腺组织明显减少(P<0.05),且乳腺癌组织中CDlc+DC细胞的数量比S-100+DC细胞的数量减少更明显,HLA-DR+DC与CD4+T细胞的数量呈明显相关关系(r=0.998);结论树突状细胞表面标志分子在人乳腺癌组织中的表达总量下降,树突状细胞在抗乳腺癌的免疫反应中可能起重要作用;这为临床开展乳腺癌的生物治疗提供了实验依据。
Objective To observe the changes of the number and expression of CDlc, S-100 and HLA-DR positive cells in human breast cancer and their relationship with T cells so as to understand the immune status of the local microenvironment in breast cancer and to explore the relationship between dendritic cells and breast The occurrence and development of cancer provide experimental evidence for the biological treatment of breast cancer. Methods Twenty cases of breast cancer were collected from surgically resected human breast cancer tissues and adjacent normal breast tissues. Immunohistochemistry and image analysis were used to detect breast cancer specimens. Results The expressions of CDlc, S-100 and HLA-DR in breast cancer tissues were significantly lower than those in normal breast tissues (P <0.05). The expressions of CD1c + DC, S-100 + DC, HLA- DR + DC and CD4 + T The number of CD13 + cells and CD8 + T cells in breast cancer tissues were significantly lower than those in normal breast tissues (P <0.05). The number of CD1c + DCs in breast cancer tissues was significantly decreased than that in S-100 + DCs. DC and CD4 + T cells was significantly correlated (r = 0.998) .Conclusion Dendritic cell surface marker molecules in human breast cancer decreased the total amount of expression of dendritic cells in anti-breast cancer immune response May play an important role; which provides experimental evidence for the clinical treatment of breast cancer.