目的:探讨快速分离小鼠颈总动脉血管平滑肌细胞(CCAVSMCs)的实验方法。方法:分离小鼠颈总动脉,用酶液Ⅰ(木瓜蛋白酶等)、酶液Ⅱ(Ⅱ型胶原酶)消化获取平滑肌细胞,并确定两步反应中酶各自的最佳消化时间,以台盼蓝染色测定细胞活性,采用免疫荧光法进行细胞鉴定。结果:在倒置显微镜下,观察并计数分离的平滑肌细胞数,酶液Ⅰ、酶液Ⅱ的消化时间分别为25min、45min时平滑肌细胞数量较多,达(50±1.3)个/低倍视野,与其他各时间点比较差异具有统计学意义(P<0.05),98%的台盼蓝染色细胞为活细胞,免疫荧光法鉴定为平滑肌细胞。结论:该方法操作简单、成本低,可在较短时间内获取数量可观、形态佳、高纯度的颈总动脉平滑肌细胞。 Objective: To investigate the method of rapid isolation of the common carotid artery smooth muscle cells (CCAVSMCs) in mice. Methods: The common carotid arteries of mice were isolated and the smooth muscle cells were obtained by digestion with enzyme solution Ⅰ (papain, etc.) and enzyme solution Ⅱ (type Ⅱ collagenase), and the optimal digestion time of each enzyme in the two-step reaction was determined. Cell viability was determined by blue staining and cell identification was performed by immunofluorescence. Results: Under inverted microscope, the number of isolated smooth muscle cells was observed. The digestion time of enzyme Ⅰ and enzyme Ⅱ were 25min and 45min, respectively. The number of SMCs was (50 ± 1.3) / low power, Compared with other time points, the difference was statistically significant (P <0.05), 98% of trypan blue-stained cells were viable cells, and the immunofluorescence method was identified as smooth muscle cells. Conclusion: The method is simple, low cost and can obtain a large number of smooth muscle cells of carotid artery with good morphology and purity in a short time.