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探讨菊苣酸(Chicoric acid,CRA)在人肝细胞系HepG2细胞中对细胞因子信号转导抑制分子3(Suppressor of cyto-kine signaling 3,SOCS3)表达的影响,用剂量为0、5和50μg.mL-1 CRA分别处理HepG2细胞24h后,经RT-PCR、Real-time PCR检测SOCS3基因的mRNA表达情况,再以上述不同剂量CRA分别处理细胞24h以及50μg.mL-1CRA分别处理HepG2细胞0、6、12、18和24h后,用Western blot法检测SOCS3基因的表达情况。研究结果显示CRA作用于HepG2细胞后,SOCS3基因的mRNA和蛋白表达水平随着CRA剂量增加而显著下降(p<0.01),且呈现一定的剂量依赖性;随着处理时间的增加,SOCS3蛋白表达水平下降更加明显,说明在翻译水平CRA不仅呈剂量依赖性,还呈时间依赖性下调SOCS3蛋白的表达。这些结果提示CRA可抑制SOCS3蛋白在HepG2细胞中的表达。
To investigate the effect of cholic acid (CRA) on the expression of suppressor of cyto-kine signaling 3 (SOCS3) in human hepatoma cell line HepG2 at doses of 0, 5 and 50 μg. mL-1 CRA for 24 h, respectively. The expression of SOCS3 mRNA in HepG2 cells was detected by RT-PCR and Real-time PCR. The HepG2 cells were treated with different doses of CRA for 24 h and 50 μg.mL- After 6, 12, 18 and 24h, the expression of SOCS3 gene was detected by Western blot. The results showed that the expression of SOCS3 mRNA and protein in HepG2 cells decreased significantly with the increase of CRA dosage (p <0.01), and showed a dose-dependent manner. With the increase of treatment time, SOCS3 protein expression The level of CRP was more obvious, indicating that CRA not only in a dose-dependent manner, but also down-regulated SOCS3 protein expression in a time-dependent manner. These results suggest that CRA inhibits the expression of SOCS3 protein in HepG2 cells.