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目的研究巴曲酶注射液对大鼠血栓栓塞性脑卒中急性超早期的保护作用。方法自体血凝块闭塞左侧大脑中动脉法制备大鼠血栓栓塞性脑卒中模型,32只造模成功大鼠按神经缺陷程度随机分为4组:模型组及巴曲酶注射液低、高剂量(0.3、1.0 BU/kg)组和阿替普酶(rt-PA,9 mg/kg)组,每组8只,另设假手术组8只。造模1 h后尾iv给药,给药后6 h行神经功能评分,采用核磁共振(MIR)技术进行大鼠脑SE-T2WI序列扫描,测量脑病变范围;给药后24 h评分后取脑进行TTC染色,测量脑梗死范围;给药后6、24 h取血浆,测纤维蛋白原(FIB)浓度。结果与模型组比较,巴曲酶注射液0.3 BU/kg治疗24 h(P<0.05)、1 BU/kg治疗6、24 h(P<0.05、0.01)显著改善大鼠神经功能评分;给药后6 h MRI结果显示,巴曲酶注射液0.3、1.0 BU/kg显著缩小病变范围(P<0.05、0.01);给药后24 h TTC结果显示,巴曲酶注射液0.3、1.0 BU/kg显著缩小梗死范围(P<0.05);巴曲酶注射液0.3、1.0 BU/kg于药后6、24 h均可显著降低血浆FIB浓度(P<0.05、0.01、0.001)。结论巴曲酶注射液能改善大鼠脑缺血急性期受损神经功能、缩小脑病变范围、降低血浆FIB浓度,具脑保护作用。
Objective To study the protective effect of batroxobin on the acute and early stage of thromboembolic stroke in rats. Methods Thromboembolism model of thromboembolism was established by occlusion of left middle cerebral artery with blood clot. 32 rats were randomly divided into 4 groups according to their degree of neurological deficit. The model group and batroxobin injection were low and high The rats in each group were treated with 0.3, 1.0 BU / kg and alteplase (rt-PA, 9 mg / kg), with 8 in each group. At the end of 1 h after model administration, the neurological function score was evaluated 6 h after the administration, and the SE-T2WI sequence scan was performed by using the nuclear magnetic resonance (MIR) technique to measure the range of brain lesions. After 24 h, Brain TTC staining, measurement of cerebral infarction range; 6,24 h after administration of plasma, fibrinogen (FIB) concentration. Results Compared with model group, Batroxobin injection at a dose of 0.3 BU / kg for 24 h (P <0.05) and 1 BU / kg treatment for 6 and 24 h significantly improved neurological function scores (P <0.05, 0.01) Six hours later, the results of MRI showed that Batroxobin 0.3,1.0 BU / kg significantly reduced the range of lesions (P <0.05, 0.01); TTC 24 h after administration showed that Batroxobin 0.3, 1.0 BU / kg (P <0.05). Batroxobin 0.3,1.0 BU / kg significantly decreased plasma FIB concentrations at 6 and 24 h (P <0.05, 0.01 and 0.001), respectively. Conclusion Batroxobin can improve neurological deficits in acute stage of cerebral ischemia in rats, reduce the range of brain lesion, decrease the concentration of FIB in the brain and protect the brain with cerebral protection.