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目的探讨５ｑ微卫星不稳定性（ＭＳＩ）与ＡＰＣ／ＭＣＣ基因杂合缺失（ＬＯＨ）的关系。方法应用ＰＣＲ－ＳＳＬＰ及ＰＣＲ－ＲＦＬＰ技术分析５２例手术切除胃癌组织中ＭＳＩ及ＡＰＣ／ＭＭＣ基因ＬＯＨ。结果５ｑＭＳＩ检出率为３４．０％（１６／４７），ＡＰＣ／ＭＣＣ基因ＬＯＨ率为３１．４％（１１／３５）。早期胃癌５ｑＭＳＩ阳性率为６６．７％（２／３），ＡＰＣ／ＭＣＣＬＯＨ率为５０％（１／２）；进展期分别为３１．８（１４／４４），３０．３％（１０／３３）。两组间无显著差别（Ｐ＞０．０５）。ＭＳＩ及杂合缺失与肿瘤大小、浸润深度、淋巴结转移及临床分期无关。粘液（印戒）细胞癌ＡＰＣ／ＭＣＣＬＯＨ率（５５．６％）显著高于高、中分化管状腺癌（Ｐ＜０．０５）。胃、肠两型胃癌５ｑＭＳＩ及ＡＰＣ／ＭＣＣＬＯＨ差异无显著性及５ｑＭＳＩ与ＡＰＣ／ＭＣＣＬＯＨ无相关性（Ｐ＞０．０５）。结论染色体５ｑＭＳＩ有ＡＰＣ／ＭＣＣ基因ＬＯＨ在两型胃癌的早期发生及发展中起一定作用。染色体５ｑ可能是胃癌的易感部位。
Objective To investigate the relationship between 5Q microsatellite instability (MSI) and heterozygous deletion of APC/MCC gene (LOH). Methods PCR-SSLP and PCR-RFLP techniques were used to analyze the loci of MSI and APC/MMC genes in 52 surgically resected gastric cancer tissues. Results The detection rate of 5qMSI was 34.0% (16/47), and the LOH rate of APC/MCC gene was 31.4% (11/35). The positive rate of 5qMSI in early gastric cancer was 66.7% (2/3), the APC/MCCLOH rate was 50% (1/2), and the progressive period was 31.8 (14/44) and 30.3% (10/33) respectively. ). There was no significant difference between the two groups (P>0.05). Loss of MSI and heterozygosity was not associated with tumor size, depth of invasion, lymph node metastasis, and clinical stage. The rate of APC/MCCLOH (55.6%) in mucinous (signet ring) cell carcinoma was significantly higher than that of high and moderately differentiated tubular adenocarcinoma (P<0.05). Gastric and intestinal type 5qMSI and APC/MCCLOH had no significant difference, and 5qMSI had no correlation with APC/MCCLOH (P>0.05). Conclusion The chromosome 5qMSI has APC/MCC gene LOH plays an important role in the early stage of the development of two types of gastric cancer. Chromosome 5q may be a susceptible site of gastric cancer.