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通过开顶式CO2气室研究了盛蕾期转Bt棉花新棉33B及其对照亲本DP5415的生长势和氮素代谢特征对土壤氮素水平(100和200mgN.kg-1)和CO2浓度倍增(750和375μl.L-1)的生理生态响应。结果表明:CO2浓度升高可显著提高2种棉花的株高和茎粗,增加生物产量;氮素水平提高可显著增加转Bt棉花的株高、茎粗,以及茎和蕾的鲜质量,而对亲本棉花DP5415的影响不显著;对照棉花DP5415的谷氨酰胺合成酶(GS)活力随大气CO2浓度的升高而显著降低,随氮素营养的提高而升高,转Bt棉花新棉33B在低氮条件下,GS活力随大气CO2浓度的升高而显著增加;大气CO2浓度升高及氮素营养的增加使盛蕾期转Bt棉花的硝酸还原酶(NR)活力显著增加,DP5415的NR活力也随大气CO2浓度的升高而显著提高;大气CO2浓度对2种棉花的亚硝酸还原酶(NiR)活力都有明显的抑制作用,其中,高CO2浓度条件下,DP5415的NiR活力还随氮素营养的增加而显著下降。可见,大气CO2浓度升高下,土壤氮素水平变化对转Bt棉花的生长势影响显著,但对其氮素代谢生理的影响较对照亲本棉花小。生产中(尤其是高浓度CO2环境下),应进一步加强转Bt棉花的氮肥优化管理。
The effects of nitrogen balance (100 and 200 mg N · kg-1) and doubled CO2 concentration (Bt cotton) on the growth potential and nitrogen metabolism of Bt cotton new cotton 33B and its parent DP5415 750 and 375 μl.L-1). The results showed that: the increase of CO2 concentration could significantly increase the plant height and stem diameter of two kinds of cotton and increase the biological yield; the increase of nitrogen level could significantly increase the plant height, stem diameter, fresh weight of stem and bud, The effect of DP5415 on cotton DP5415 was not significant. The activity of glutamine synthetase (GS) of cotton DP5415 was significantly decreased with the increase of atmospheric CO2 concentration and increased with the increase of nitrogen nutrition. Under low nitrogen conditions, GS activity increased significantly with the increase of atmospheric CO2 concentration. The increase of atmospheric CO2 concentration and nitrogen nutrition increased the nitrate reductase (NR) activity of Bt cotton in Shenglei stage, The vitality also increased significantly with the increase of atmospheric CO2 concentration. The atmospheric CO2 concentration significantly inhibited the activity of nitrite reductase (NiR) of both cottons. Under high CO2 concentration, the NiR activity of DP5415 also decreased Nitrogen nutrition increased significantly decreased. It can be seen that under the increase of atmospheric CO2 concentration, the change of soil nitrogen level had a significant effect on the growth potential of transgenic Bt cotton, but had less impact on its nitrogen metabolism physiology than that of the control parent cotton. In production (especially in high-CO2 environment), the nitrogenous fertilizer management of Bt cotton should be further strengthened.