目的探讨鞣花酸诱导HEK-293细胞DNA损伤与氧化应激的关系。方法鞣花酸处理HEK-293细胞后,运用彗星试验检测细胞DNA损伤情况;免疫组化方法检测细胞内8-羟基脱氧鸟苷(8-OHdG)表达水平;紫外分光光度计测定细胞内活性氧(ROS)及还原性谷胱甘肽(GSH)水平。结果 120μmol/L鞣花酸作用1 h能够引起细胞彗星样拖尾,彗星尾部DNA%、尾长、尾矩分别为(12.34±4.21)%、(20.76±3.59)μm和(3.16±0.74),均高于对照组;细胞内ROS含量明显增加(P<0.01),GSH水平下降(P<0.05),60、120μmol/L鞣花酸作用3 h后均引起细胞内8-OHdG表达水平升高(P<0.01)。结论鞣花酸处理HEK-293细胞后使细胞氧化与抗氧化系统失衡,氧化应激引起的氧化性损伤可能是鞣花酸致细胞DNA损伤的重要原因之一。 Objective To investigate the relationship between DNA damage and oxidative stress induced by ellagic acid in HEK-293 cells. Methods HEK-293 cells were treated with ellagic acid, and the DNA damage was detected by comet assay. The expression of 8-OHdG was detected by immunohistochemistry. The levels of intracellular reactive oxygen species (ROS) and reducing glutathione (GSH) levels. Results Elongation of comet cells was induced by 120μmol / L ellagic acid for 1 h. The DNA%, tail length and tail moment of comet tail were (12.34 ± 4.21)%, (20.76 ± 3.59) μm and (3.16 ± 0.74), respectively (P <0.01), while the level of GSH decreased (P <0.05). After 60 and 120μmol / L ellagic acid treatment, the expression of 8-OHdG increased (P <0.01). Conclusions The oxidative damage caused by oxidative stress may be one of the important causes of DNA damage induced by ellagic acid in HEK-293 cells.