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目的探讨硒和锌对小鼠成釉细胞DNA损伤的保护作用。方法以2.50、5.00、10.00μmol/L亚硒酸钠用于小鼠成釉细胞为硒作用组,以5.00、10.00、20.00μmol/L硫酸锌作用于小鼠成釉细胞为锌作用组。细胞作用24 h后,采用单细胞凝胶电泳技术(SCGE)检测DNA单链断裂情况。结果与对照组比较,2.50和5.00μmol/L亚硒酸钠作用组小鼠成釉细胞DNA损伤的指标尾长、Olive尾矩、尾DNA%和尾长/头长值均明显下降(P<0.05);10.00μmol/L亚硒酸钠作用组小鼠成釉细胞的尾长、尾/头长比值较对照组均显著性降低(P<0.05)。与对照组比较,5.00、10.00和20.00μmol/L硫酸锌作用组可使小鼠成釉细胞尾长和Olive尾距明显下降,差异均有统计学意义(P<0.05);5.00和10.00μmol/L硫酸锌作用组尾长/头长值明显下降(P<0.05)。以Olive尾矩为观察指标,2.50和5.00μmol/L亚硒酸钠的保护作用要优于10.00μmol/L亚硒酸钠,以2.50μmol/L亚硒酸钠保护作用相对较好;10.00和20.00μmol/L硫酸锌的保护作用要优于5.00μmol/L硫酸锌,以10.00μmol/L硫酸锌保护作用相对较好。结论 2.50~10.0μmol/L的亚硒酸钠和5.00~20.00μmol/L硫酸锌均对成釉细胞DNA损伤具有一定的保护作用,其中2.50μmol/L亚硒酸钠和10.00μmol/L硫酸锌的相对保护作用较好。
Objective To investigate the protective effects of selenium and zinc on DNA damage of ameloblasts in mice. Methods Sodium selenite (2.50, 5.00 and 10.0 μmol / L) was used as selenium in mouse ameloblasts. Zinc sulfate (5.00, 10.0, 20.00 μmol / L) After 24 hours of cell function, single strand gel electrophoresis (SCGE) was used to detect DNA single strand breaks. Results Compared with the control group, the tail length, Olive tail moment, tail DNA% and tail length / head length of ameloblasts in 2.50 and 5.00μmol / L sodium selenite groups were significantly decreased (P < 0.05). The tail length, tail length / head length ratio of ameloblasts in 10.00μmol / L sodium selenite group were significantly lower than those in control group (P <0.05). Compared with the control group, 5.00, 10.00 and 20.00μmol / L zinc sulfate groups significantly decreased tail length and Olive tail of ameloblasts in both groups (P <0.05); 5.00 and 10.00μmol / L zinc sulfate group tail length / head length decreased significantly (P <0.05). Olive tail moment as an indicator, 2.50 and 5.00μmol / L sodium selenite protective effect is better than 10.00μmol / L sodium selenite, 2.50μmol / L sodium selenite protective effect is relatively good; 10.00 and The protective effect of 20.00μmol / L zinc sulphate is better than that of 5.00μmol / L zinc sulphate, and the protective effect of 10.00μmol / L zinc sulphate is relatively good. Conclusion 2.50 ~ 10.0μmol / L sodium selenite and 5.00 ~ 20.00μmol / L zinc sulfate all have a protective effect on DNA damage of ameloblasts, among them 2.50μmol / L sodium selenite and 10.00μmol / L zinc sulfate The relative protection is better.