1986年以来我们对4000余张切片做了多种免疫组化染色,其中绝大多数是用PAP法(peroxidase-anti-peroxidase complex)。经方法改进后结果满意,背景清晰,阳性颗粒清楚,总结如下: 1 标本:系1963～1988年经10％福尔马林常规固定处理涉及绒毛膜癌、恶性葡萄胎和葡萄胎的人体病理档案蜡块,每个蜡块连续切片5～30张,4μm厚,以妊娠3个月正常绒毛为阳性对照,省略1抗、2抗和PAP为阴性对照。 Since 1986, we have performed a variety of immunohistochemical staining on more than 4,000 sections, most of them using the peroxidase-anti-peroxidase complex. After the method is improved, the results are satisfactory, the background is clear, and the positive particles are clear. The results are summarized as follows: 1 Specimens: Department of Human Pathology, involving choriocarcinoma, malignant mole and hydatidiform mole fixed by 10% formalin in 1963-1988 Paraffin block, each section of wax 5 to 30 consecutive slices, 4μm thick, normal villi 3 months of pregnancy as a positive control, omitted 1 anti-2 and PAP negative control.