用放射免疫法测定缓激肽(BK)和P物质(SP)含量,发现正常大鼠(B/N-Ki)左后肢用47℃,热水刺激20 min,后肢足跖皮下灌流液中BK和SP含量分别为43±34和11.1±9.7fmol·min~(-1),比热刺激前显著增加,但在血浆激肽原缺乏大鼠(B/N-Ka),BK和SP含量分别为1.3±1.0和5.5±3.5fmol·min~(-1),明显低于B/N-Ki大鼠。B/N-Ka大鼠的E-vans蓝漏出量也低于B/N-Ki大鼠。表明热刺激时产生的BK可介导SP的释放。 Radioimmunoassay was used to determine the content of bradykinin (BK) and substance P (SP) in the left hind limbs of normal rats (B / N-Ki) And SP were 43 ± 34 and 11.1 ± 9.7 fmol · min ~ (-1), respectively, which were significantly increased before heat stimulation. However, the content of BK and SP in plasma kallikrein-deficient rats (B / N-Ka) 1.3 ± 1.0 and 5.5 ± 3.5fmol · min ~ (-1), respectively, which was significantly lower than that of B / N-Ki rats. E-vans blue leakage was also lower in B / N-Ka rats than in B / N-Ki rats. This indicates that BK produced during thermal stimulation mediates the release of SP.