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目的明确浙江湖州解放军第98医院临床分离的耐β内酰胺类抗生素鲍曼不动杆菌中Ⅰ类整合酶基因(int Ⅰ 1)及氨基糖苷类修饰酶(aminoglycoside-modifying enzymes,AMEs)基因分布状况。方法采用聚合酶链反应(PCR)及序列分析的方法分析 int Ⅰ 1及6种 AMEs 基因 aac(3)-Ⅰ、aac(3)-Ⅲ、aac(6′)-Ⅰ、ant(3″)-Ⅰ、ant(2″)-Ⅰ和 aph(3′)-Ⅵ。结果鲍曼不动杆菌对头孢噻吩完全耐药,亚胺培南、美洛培南及氨基糖苷类耐药率分别为1.7%、1.7%和86.7%。60株菌中,int Ⅰ 1、aac(3)-Ⅰ、aac(6′)-Ⅰ、ant(3″)-Ⅰ、ant(2″)-Ⅰ和 aph(3′)-Ⅵ基因 PCR 扩增阳性株数(%)分别为35(58.3%)、31(51.7%)、33(55.0%)、36(60.0%)、12(20.0%)和2(3.3%),aac(3)-Ⅲ均阴性。结论解放军第98医院临床分离的耐β内酰胺类鲍曼不动杆菌多重耐药严重,int Ⅰ 1及 AMEs 基因携带率均较高。
Objective To determine the gene distribution of int Ⅰ 1 and aminoglycoside-modifying enzymes (AMEs) in clinically isolated β-lactam antibiotic-resistant Acinetobacter baumannii isolates from the 98th Hospital of Huzhou People’s Liberation Army, Zhejiang Province. . Methods The gene sequences of aac (3) -Ⅰ, aac (3) -Ⅲ, aac (6 ’) - Ⅰ, ant (3 “) were amplified by polymerase chain reaction (PCR) and sequence analysis. -I, ant (2 ”) - I and aph (3 ’) - VI. Results Acinetobacter baumannii was completely resistant to cefalotin. The resistance rates of imipenem, meropenem and aminoglycosides were 1.7%, 1.7% and 86.7%, respectively. Among 60 isolates, the genes of int Ⅰ 1, aac (3) - Ⅰ, aac (6 ’) - Ⅰ, ant (3 “) - Ⅰ, ant (2”) - Ⅰ and aph (3’) - The number of positive cells was 35 (58.3%), 31 (51.7%), 33 (55.0%), 36 (60.0%), 12 (20.0%) and 2 (3.3% All negative. Conclusion The clinically isolated β-lactamase-resistant Acinetobacter baumannii isolated from the 98th Hospital of People’s Liberation Army has a serious multi-drug resistance. The carriage rates of int Ⅰ 1 and AMEs are high.