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苹果金冠品种的休眠茎尖经３种不同方法（玻璃化法、包埋－干燥法和两步降温法）超低温保存后，均获得成功，存活率达到了８３％。休眠茎尖由于经受了冬季的深冷驯化和具有较低的含水量，因而成为超低温保存的良好试材。经过玻璃化液（ＰＶＳ３：５０％甘油＋５０％蔗糖）预处理８０ｍｉｎ（２５℃条件下）后或经蔗糖溶液预培养并于无菌空气中干燥脱水至含水量３０％左右后，休眠茎尖可直接投入液氮进行超低温保存。保存后的休眠茎尖经离体培养后可直接再生植株。试验进一步验证了试材生理状态的选择是超低温保存成功的一个关键因素，这不仅可以简化超低温保存的程序，而且可以提高此方法的实用性，为早日建立植物种质的超低温保存库奠定了基础。
The dormant shoot tips of apple golden crown varieties were successfully obtained after cryopreservation by three different methods (vitrification, embedding-drying and two-step cooling) with a survival rate of 83%. Dormant stem tips are well tested for cryogenic preservation due to their winter acclimation and low water content. After dormancy (PVS3: 50% glycerol + 50% sucrose) pretreatment 80min (25 ℃) or pre-cultured with sucrose solution and dried in sterile air to about 30% Directly into liquid nitrogen for cryogenic preservation. After dormant dormant stem tips can be regenerated directly after in vitro culture. The test further verified that the selection of the physiological state of the test material is a key factor for the success of cryopreservation, which not only simplifies the procedure of cryopreservation, but also improves the practicability of the method and lays the foundation for the early establishment of cryopreservation of plant germplasm .