Aim:To examine the effect of curcumin on oxidative DNA damage and cell apoptosisand injury caused by the reaction of methylglyoxal(MG)with amino acids.Methods:We used DNA strand breaks to examine the effect of curcumin on oxidative DNAdamage.In addition,reactive oxygen species(ROS)formation occurs in MG-treatedmononuclear cells,so the effect of curcumin on ROS generation was measuredusing 2′,7′-dichlorofluorescin diacetate(DCF-DA)as the detection reagent.Moreover,the impact effects of curcumin on MG-induced cell apoptosis and ROSinjury were analyzed by TUNEL and ELISA assay.The collagen Ⅰ attachmentability of mononuclear cells was examined by trypan blue staining.Results:Ourresults revealed that curcumin prevented MG/lysine-induced oxidative stress andDNA damage.Curcumin also inhibited MG-induced apoptosis and generation ofROS in mononuclear cells.MG-treated mononuclear cells displayed a lowerdegree of attachment to collagen(the major component of the vessel wall subendo-thelium),whereas cells pretreated with curcumin before MG treatment exhibitedrestored affinities for collagen.Conclusion:These results demonstrated thatoxidative stress plays a role in MG-induced cell injury and alterations in attach-ment ability,and that curcumin blocks these effects by virtue of its antioxidantproperties. Aim:To examine the effect of curcumin on oxidative DNA damage and cell apoptosisand injury caused by the reaction of methylglyoxal(MG)with amino acids.Methods:We used DNA strand breaks to examine the effect of curcumin on oxidative DNAdamage.In addition,reactive Oxygen species(ROS) formation occurs in MG-treated mononuclear cells, so the effect of curcumin on ROS generation was measured using 2′,7′-dichlorofluorescin diacetate(DCF-DA)as the detection reagent.Moreover, the impact effects of curcumin on MG -induced cell apoptosis and ROSinjury were analyzed by TUNEL and ELISA assay.The collagen I attachmentability of mononuclear cells was examined by trypan blue staining.Results:Ourresults revealed that curcumin prevented MG/lysine-induced oxidative stress andDNA damage.Curcumin also inhibited MG- Induced apoptosis and generation ofROS in mononuclear cells.MG-treated mononuclear cells displayed a lowerdegree of attachment to collagen(the major component of the vessel wall subendo-thelium) , Whereas cells pretreated with curcumin before MG treatment exhibitedorred affinities for collagen.Conclusion:These results reveal thatoxidative stress plays a role in MG-induced cell injury and alterations in attach-ment ability, and that curcumin blocks these effects by virtue of its antioxidantproperties.