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发根农杆菌 (Agrobacteriumrhizogenes)R16 0 1感染商陆叶片外植体 1周后 ,在其切口处产生毛状根 ,2 0d后产生毛状根的外植体比例达 70 % ;毛状根可直接从叶片外植体叶脉处或从叶脉处产生的愈伤组织上产生。毛状根能在无激素的MS培养基上自主生长 ,其呼吸速率比对照根提高 85 6 %。冠瘿碱检测和PCR扩增结果表明 ,发根农杆菌RiT DNA的冠瘿碱合成酶基因及其Ri质粒的rol基因均已在商陆毛状根基因组中得到表达。毛状根中总皂甙含量约为自然根的 1 5 4倍 ,但其多糖含量则仅为非转化根的 70 %。
After 1 week of Agrobacterium rhizogenes R16 0 1 infection, the hairy roots were produced at the incision and 70% of the explants with hairy roots after 20 days. The hairy roots Generated directly from the leaf explants of the leaf explants or from the callus generated at the leaf veins. Hairy roots can grow autonomously on hormone-free MS medium with a respiration rate of 85.6% higher than that of control roots. The results of amphotericin assay and PCR amplification indicated that the rol gene of the triumphine synthase gene of RiT DNA of A. rhizogenes and its Ri plasmid was expressed in the hairy root genomic genome. The content of total saponin in hairy root is about 154 times that of natural roots, but its polysaccharide content is only 70% of that of non-transformed roots.