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目的探讨慢性铅中毒导致的模型大鼠学习记忆能力损伤及中药的干预作用。方法 60只大鼠随机分为2组,其中空白组12只,饮用双蒸水;造模组48只,饮含0.02%醋酸铅的双蒸水,连续60 d后将造模动物随机分为4组:高剂量治疗组(按3.0 g·kg-1·d-1的剂量灌胃自制中药颗粒)、低剂量治疗组(0.6 g·kg-1·d-1)、阳性对照组(依地酸钠钙加普鲁卡因肌肉内注射,50 mg·kg-1·d-1)及模型组(不予任何治疗),连续60 d后进行隐藏站台试验及空间探索试验,采血检测血铅,RT-PCR检测脑组织海马区NR1、NR2B m RNA表达。结果经统计,与模型组比较,其他各组体重、血色素、穿过平台次数、第3象限活动时间、NR1、NR2B m RNA表达均显著增加(P<0.05),血铅含量、Morris水迷宫试验d 4潜伏期显著降低(P<0.05);与阳性对照组比较,中药高剂量组体重、第3象限活动时间、NR1m RNA表达、NR2B m RNA表达显著增加(P<0.05)。结论自制中药颗粒通过增加脑组织海马区NR1 m RNA、NR2B m RNA的表达,改善慢性铅中毒导致的学习记忆能力损伤。
Objective To investigate the impairment of learning and memory ability induced by chronic lead poisoning and the intervention effect of traditional Chinese medicine (TCM). Methods Sixty rats were randomly divided into two groups, of which 12 rats in the blank group were drank with distilled water and 48 rats in the model group were drank twice with distilled water containing 0.02% lead acetate. The animals were randomly divided into 4 groups Group: high-dose treatment group (at the dosage of 3.0 g · kg-1 · d-1 gavage homemade Chinese medicine particles), low-dose treatment group (0.6 g · kg -1 · d -1), positive control group Soda lime plus procaine intramuscular injection, 50 mg · kg-1 · d-1) and model group (without any treatment) for 60 days after the hidden platform test and space exploration test, blood lead test The expression of NR1 and NR2B m RNA in hippocampus of brain tissue was detected by RT-PCR. Results Compared with the model group, the body weight, hemoglobin, the number of passing through the platform, the time of the third quadrant, the expression of NR1 and NR2B m RNA in the other groups were significantly increased (P <0.05), and the levels of blood lead, Morris water maze test (P <0.05). Compared with the positive control group, the body weight, the time of the third quadrant, the expression of NR1mRNA and the expression of NR2B m RNA in the high dose group increased significantly (P <0.05). CONCLUSION: Self-made Chinese medicine granules can improve the learning and memory impairment caused by chronic lead poisoning by increasing the expression of NR1 m RNA and NR2B m RNA in hippocampus of brain tissue.