肠球菌引起的感染及院内感染问题近年来备受国内外有关部门重视。控制感染的一个重要策略就是弄清传染源及传播途径 ,中断菌株的传播 ,这样的流行病学研究依赖于有高鉴别力的分型系统来辨别菌株的相关性。本文概述了PFGE法、RAPD法、质粒分型、核糖分型及限制性内切酶分型等技术对肠球菌分型的研究 ,对上述各法的优缺点进行了分析及展望。 Enterococci caused infections and nosocomial infections in recent years, much attention by relevant departments at home and abroad. An important strategy for infection control is to understand the source of infection and the route of transmission, interrupting the spread of the strain, and such epidemiological studies rely on highly discriminating typing systems to discern the strain’s association. This article summarizes the PFGE method, RAPD method, plasmid typing, ribotyping and restriction endonuclease typing of enterococci typing, the advantages and disadvantages of the above methods were analyzed and prospects.