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以果蔗(Badila)茎尖脱毒组培苗为材料,利用间歇浸没式生物反应器(Temporary immersion bioreactors TIBs)进行组培快繁的技术体系的研究。结果表明:(1)使用TIBs系统进行甘蔗组织培养一代增殖较传统方法高出10倍以上;(2)TIBs系统以第4代继代材料为宜;(3)接种密度在10～15株/L培养基最有利于甘蔗组培苗的增殖和生长;(4)TIBs系统中6-BA浓度为0.5～1.0mg/L时适合于增殖培养,NAA浓度为4mg/L时有利于根的诱导;(5)浸没间歇频率在1min/3h时增殖和生长表现较为优异,1min/6h时有利于根的诱导和根的生长。
Tissue culture techniques were studied using Tissues dendritic cells (TIBs) from Tissues of Badila shoot tips. The results showed that: (1) TIBs system was used to propagate more than ten times more than the traditional method; (2) TIBs system was the fourth generation of subculture materials; (3) Inoculation density of 10-15 plants / L medium was the most suitable for the proliferation and growth of sugarcane tissue culture seedlings. (4) When the concentration of 6-BA was 0.5-1.0mg / L in TIBs system, it was suitable for proliferation culture. When NAA concentration was 4mg / L, ; (5) The intermittent frequency of immersion showed better proliferation and growth at 1 min / 3 h, which was beneficial to root induction and root growth at 1 min / 6 h.