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目的:建立高效液相色谱法同时测定补肾益寿片中朝藿定A、朝藿定B、朝藿定C和淫羊藿苷的含量。方法:采用EliteKromasil C18(4.6 mm×250 mm,5μm)色谱柱,流动相为乙腈(A)-水(B),梯度洗脱(0～24 min,25%A→27%A;24～30 min,27%A→100%A),流速1 mL.min-1,检测波长270 nm,柱温20℃。结果:朝藿定A进样量在0.096～0.768μg,朝藿定B在0.200～1.60μg,朝藿定C在0.276～2.21μg,淫羊藿苷在0.416～3.33μg范围内呈良好线性关系;相关系数分别为0.9991,0.9986,1.0000,0.9997。朝藿定A、朝藿定B、朝藿定C和淫羊藿苷的平均回收率(n=5)分别为97.8%,99.5%,99.2%,100.2%;RSD分别为2.4%,3.9%,3.1%,3.5%。结论:本文方法可作为补肾益寿片中多指标成分检测的方法,为补肾益寿片的质量控制提供依据。
OBJECTIVE: To establish a method for the simultaneous determination of epimedin A, epimedin B, epimedin C and icariin in Bushen Yishou Tablets by high performance liquid chromatography. Methods: The mobile phase consisted of acetonitrile (A) -water (B) and gradient elution (0-24 min, 25% A → 27% A; 24-30) using an EliteKromasil C18 (4.6 mm × 250 mm, min, 27% A → 100% A), flow rate 1 mL.min-1, detection wavelength 270 nm, column temperature 20 ℃. Results: The injection of epimedin A was in the range of 0.096 ~ 0.768μg, the epimedin B was in the range of 0.200 ~ 1.60μg, the epimedin C was in the range of 0.276 ~ 2.21μg and icariin was in the range of 0.416 ~ 3.33μg ; Correlation coefficient were 0.9991,0.9986,1.0000,0.9997. The average recoveries of epimedin A, epimedin B, epimedin C and icariin were 97.8%, 99.5%, 99.2% and 100.2%, respectively; the RSDs were 2.4% and 3.9% , 3.1%, 3.5%. Conclusion: This method can be used as a method to detect multi-index components in Bushen Yishou Tablets and provide the basis for the quality control of Bushen Yishou Tablets.