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通过IS-PCR和rep-PCR指纹技术,分析了广东水稻白叶枯病菌(Xanthomonas oryzae pv.oryzae)群体遗传多样性。用2对特异性引物J3和ERIC对114个菌株基因组DNA进行了PCR扩增,分别呈现89和40种谱型,以彼此间的带位相似率达70%为界,J3扩增的谱型被分为11簇,ERIC的谱型被分为8簇。J3的簇1包含89个菌株,占总数的78.07%,ERIC的簇1包含52个菌株,占总数的45.61%,均为优势簇群。群体遗传多样性值J3为0.8919,ERIC为0.8278。上述结果表明,广东水稻白叶枯病菌的遗传多样性较高。全部参试菌株接种于含有不同抗性基因近等基因系及高感品种金刚30共6个鉴别品种,被划分为6个小种(X-gd1,X-gd2,X-gd3,X-gd4,X-gd5和X-gd6),X-gd4出现频率最高,为广东省的优势小种。
The genetic diversity of Xanthomonas oryzae pv. Oryzae population in Guangdong was analyzed by IS-PCR and rep-PCR fingerprinting. The genomic DNA of 114 strains was amplified by PCR using two pairs of primers J3 and ERIC, which showed 89 and 40 genotypes, respectively. The band-by-band similarity rate was 70% Divided into 11 clusters, the ERIC patterns are divided into 8 clusters. Cluster 1 of J3 contained 89 strains, accounting for 78.07% of the total. Cluster 1 of ERIC contained 52 strains, accounting for 45.61% of the total, which were the dominant clusters. Population genetic diversity value J3 was 0.8919, ERIC was 0.8278. The above results show that the genetic diversity of the rice bacterial blight in Guangdong is high. All the tested strains were inoculated into 6 differential cultivars including Jinjiang 30, a near isogenic line with different resistance genes and a high susceptible variety, and were divided into 6 minor species (X-gd1, X-gd2, X-gd3 and X-gd4 , X-gd5 and X-gd6), X-gd4 appeared the highest frequency, which was the dominant race in Guangdong.