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目的观察β-防御素4(mouseβdefensin 4,mBD-4)在小鼠胚胎神经系统发育不同时期的基因表达及脂多糖(lipopolysaccharide,LPS)诱导孕鼠后mBD-4在胚鼠神经系统的的表达情况。方法孕鼠分为LPS组(n=10)和正常对照组(n=10),取不同时期(5.5、8.5、11.5、17.5天)小鼠胚胎,LPS组于胚胎提取1天前母鼠腹腔注射LPS,正常对照组同时间注射同量的生理盐水,胚胎取出后石蜡包埋切片,免疫组化方法和RT-PCR技术检测mBD-4的阳性表达。结果 mBD-4在小鼠胚胎早期第5.5天的神经组织中未见固有表达,到胚胎第8.5天开始有少量固有表达,到胚胎中晚期,固有表达含量增多;LPS组中胚胎神经组织中自5.5天有阳性表达,且随着胚胎的发育其阳性表达越强。结论①mBD-4在小鼠胚胎神经系统中固有表达,在胚胎第8.5天有少量表达,且随着神经系统的发育成熟,mBD-4在其神经组织中表达含量越多。③mBD-4在LPS的诱导下,其在神经组织中表达明显增高。
Objective To observe the gene expression of mBD-4 and the expression of mBD-4 in the nervous system of embryonic rat after pregnancy induced by lipopolysaccharide (LPS) Happening. Methods Pregnant mice were divided into LPS group (n = 10) and normal control group (n = 10). The mouse embryos at different time points (5.5, 8.5, 11.5, 17.5 days) LPS was injected into the normal control group. The same amount of normal saline was injected into the normal control group. The paraffin-embedded sections were removed after embryo removal. The expression of mBD-4 was detected by immunohistochemistry and RT-PCR. Results No intrinsic expression of mBD-4 was found in the neural tissue of the first 5.5 days in mouse embryos, and there was a small amount of intrinsic expression of mBD-4 in embryonic day 8.5. The intrinsic expression of mBD- 5.5 days have positive expression, and with the development of embryos, the stronger the positive expression. Conclusion ①mBD-4 is expressed naturally in the embryonic nervous system of mice, and it is expressed on the 8.5th day of the embryo. As the nervous system matures, mBD-4 is expressed more in the nervous tissues. ③mBD-4 induced by LPS, its expression in nerve tissue was significantly increased.