一、试验目的紫花蓿苜为异花授粉植物,在没有异地隔离的条件下,品种极易混杂,因此,对于紫花蓿苜的育种,特别是育种初期,采用无性芃殖方法,可以加速育种进程,并保持遗传性的稳定。1981年根据我所现有的设备条件,应用MS和N_6培养基进行了茎段培养试验,取得了较好的结果。二、试验材料和制取方法 First, the purpose of the trial alfalfa aloe cross-pollination plants, without isolation in different conditions, the species can easily be mixed, therefore, alfalfa breeding, especially in the early breeding, the use of asexual breeding method can speed up the breeding process , And maintain genetic stability. In 1981, according to my existing equipment conditions, the MS and N_6 medium were used to carry out the stem culture experiment, and the better results were obtained. Second, the test materials and preparation methods