论文部分内容阅读
1963年Vago和Bergoin报道了大粉蝶Pieris brassicae颗粒体病毒在舞毒蛾Lymantriadispar原代卵巢细胞培养物中复制,但后来一直未能重复。关于菜粉蝶Pieris rapael的组织培养国内外则很少见有报道,为了研究菜粉蝶颗粒体病毒与其宿主的关系、病毒的增殖机制和探索大量生产病毒的新途径,我们开展了菜粉蝶的细胞在体外的培养工作。菜粉蝶幼虫原代血细胞体外培养4天后形成单层,一般能维持7到10天。 材料与方法 材料 菜粉蝶幼虫是从市郊菜地甘蓝上采集。培养基成分见下表。 培养基用1NKOH溶液调pH至6.3,过滤除菌。
Vagin and Bergoin reported in 1963 that the Pieris brassicae granulosis virus replicated in the Gypsy moth Lymantriadispar primary ovarian cell culture but was never repeated. Tissue culture of Pieris rapael is seldom reported at home and abroad. In order to study the relationship between the cabbage granulosis virus and its host, the proliferation mechanism of the virus and explore new ways to produce the virus in large quantities, In vitro culture work. The cabbage butterfly larval primary blood cells form a monolayer after 4 days of in vitro culture and generally can last for 7 to 10 days. Materials and Methods Materials Cabbage butterfly larvae were collected from the vegetables in the suburbs. Medium composition in the table below. The medium was adjusted to pH 6.3 with 1N KOH solution and sterilized by filtration.