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目的研究多聚体蛋白聚糖(syndecan 1,CD138)单克隆抗体(mAb)4B3 对天然表达syndecan 1的人多发性骨髓瘤(MM)细胞XG 1 和XG 2 的生物学效应。方法采用间接免疫荧光标记和流式细胞术分析,通过4B3mAb观察XG 1和XG 2细胞膜上syndecan 1荧光标记阳性率;按常规培养方式对XG 1、XG 2进行细胞培养和台盼蓝染色记数,观察不同浓度的4B3mAb对XG 1和XG 2细胞体外生长作用。结果4B3mAb能识别XG 1 和XG 2 细胞表达的syndecan 1 分子,表达率与商品化的鼠抗人syndecan 1 mAb(BB4)相似;同时,4B3mAb与XG 1 和XG 2 细胞膜上synde can 1荧光标记阳性率基本一致,反应均呈强阳性;加入不同浓度的4B3mAb,XG 1 细胞数量2 d后开始下降,而XG 2细胞3 d后数量出现减少。结论4B3mAb抗体对XG 1 和XG 2 均具有明显的生长抑制作用,但XG 2细胞的生长抑制作用早于XG 1,可为MM的生物治疗提供新途径。
Objective To investigate the biological effects of syndecan 1, CD138 monoclonal antibody (mAb) 4B3 on human multiple myeloma (MM) cells XG 1 and XG 2 that naturally express syndecan 1. Methods Indirect immunofluorescence staining and flow cytometry analysis were used to observe the positive rate of syndecan 1 on XG 1 and XG 2 cell membranes by 4B3 mAb; Cell culture and trypan blue staining were performed on XG 1 and XG 2 cells by conventional culture methods. Observe the growth effects of different concentrations of 4B3 mAb on XG 1 and XG 2 cells in vitro. Results The 4B3 mAb recognized the syndecan 1 molecules expressed in XG 1 and XG 2 cells, and the expression rate was similar to that of the commercial mouse anti-human syndecan 1 mAb (BB4). Meanwhile, the 4B3 mAb was positive for syndecan 1 on the XG 1 and XG 2 cell membranes. The rates were basically the same and the reactions were strongly positive; adding different concentrations of 4B3 mAb, the number of XG 1 cells began to decrease after 2 days, and the number of XG 2 cells decreased after 3 days. Conclusion The 4B3 mAb antibody has obvious growth inhibitory effects on XG 1 and XG 2 , but the growth inhibitory effect of XG 2 cells is earlier than that of XG 1, which can provide a new approach for biological treatment of MM.