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目的:研究光动力学疗法对动物肿瘤生长的影响及生长抑素(somatostantin,SS)、ghrelin表达的变化。方法:选用皮下接种W256瘤细胞株制成实验性荷瘤大鼠,观察经紫外光照射、充氧并加注血卟啉的血液对大鼠移植肿瘤生长的影响,同时采用放射免疫检测方法观察血浆、组织中SS含量的改变;采用实时PCR和免疫荧光组化方法观察了ghrelinmRNA和ghrelin在脑内表达的影响。结果:荷瘤大鼠输入经紫外光照射、充氧并加注血卟啉的血液,其肿瘤生长速度明显减慢,肿瘤重量明显减轻(P<0.01)。荷瘤大鼠下丘脑、中脑、桥延脑、肝脏、瘤组织及血浆SS含量与正常大鼠比较显著增加(P<0.05),下丘脑、垂体ghrelinmRNA表达显著减少,同时下丘脑弓状核(Arc)内ghrelin免疫阳性细胞显著减少(P<0.01),但经光动力学疗法治疗肿瘤后,上述各组织和血浆中SS含量明显下降(P<0.01),下丘脑、垂体ghrelinmRNA含量和下丘脑弓状核内ghrelin免疫阳性细胞数量均显著增加(P<0.05)。结论:光动力学疗法对肿瘤生长有抑制作用;肿瘤生长过程中激活某种途径刺激机体产生生长抑素以提高机体抵抗肿瘤生长的能力,同时ghrelin也参与了肿瘤的发生和发展。
Objective: To study the effects of photodynamic therapy on tumor growth and the changes of somatostantin (SS) and ghrelin in animal models. Methods: W256 tumor cells were inoculated subcutaneously into experimental tumor-bearing rats. The effects of UV-irradiated, oxygenated and hematoporphyrin-treated blood on the growth of rat transplanted tumor were observed, and the radioimmunoassay Plasma and tissues. The effects of ghrelin mRNA and ghrelin on the brain were observed by real-time PCR and immunofluorescence staining. Results: Tumor-bearing rats were injected with UV-irradiated, oxygenated and hematoporphyrin-loaded blood, the tumor growth rate was significantly slowed down, tumor weight was significantly reduced (P <0.01). Compared with normal rats, the content of SS in hypothalamus, midbrain, medulla oblongata, liver, tumor tissue and plasma in tumor-bearing rats increased significantly (P <0.05), the expression of ghrelin mRNA in hypothalamus and pituitary decreased significantly, (P <0.01). However, the content of ghrelin in hypothalamus and pituitary gland significantly decreased after photodynamic therapy (P <0.01) The number of ghrelin immunoreactive cells in thalamus arcuate nucleus increased significantly (P <0.05). Conclusions: Photodynamic therapy can inhibit the growth of tumor. In the process of tumor growth, some pathways are activated to stimulate the body to produce somatostatin so as to enhance the ability of the body to resist tumor growth. At the same time, ghrelin is also involved in the occurrence and development of tumors.