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目的探讨抗生素与清开灵配伍前后联合抑菌效应的变化。方法以清开灵及7种常用抗生素(阿莫西林、头孢曲松钠、盐酸恩诺沙星、盐酸左氧氟沙星、氨苄西林、头孢拉定、头孢氨苄)为试验药物,采用微量二倍稀释法和棋盘稀释法分别测定清开灵及7种抗生素联用前后对金黄色葡萄球菌的最小抑菌浓度(MIC),计算联合抑菌指数(FIC),比较不同配伍的FIC值大小。结果清开灵及7种抗生素的MIC依次为5 000,0.5,1.0,1.0,0.06,0.06,0.25,0.25μg·ml~(-1);清开灵与阿莫西林、盐酸恩诺沙星、盐酸左氧氟沙星、氨苄西林、头孢氨苄联用后0.52,呈拮抗作用。结论中西药配伍后的作用效果有很大的不确定性,临床应用时最好以系统的试验数据作为依据。
Objective To investigate the changes of combined antibacterial effect before and after the combination of antibiotics and Qingkailing. Methods Qingkailing and seven commonly used antibiotics (amoxicillin, ceftriaxone sodium, enrofloxacin hydrochloride, levofloxacin hydrochloride, ampicillin, cefradine, cephalexin) as the test drug, using two-fold dilution and chessboard dilution Method were used to determine the minimum inhibitory concentration (MIC) of Qingkailing and seven antibiotics before and after the combination of Staphylococcus aureus, calculate the combined antibacterial index (FIC), compare the size of different compatibility FIC values. Results The MICs of Qingkailing and 7 antibiotics were 5 000,0.5,1.0,1.0,0.06,0.06,0.25,0.25 μg · ml -1, Qingkailing and amoxicillin, enrofloxacin hydrochloride , Levofloxacin hydrochloride, ampicillin, cefalexin combined with 0.5 2, was antagonistic. Conclusions After the compatibility of traditional Chinese and western medicine, there is a great deal of uncertainty about the effect of the combination of traditional Chinese medicine and western medicine. Based on systematic data, it is best to apply it in clinical practice.