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探讨HGV在慢性肝炎肝硬化当中感染率、临床及病理特征 ,应用ELISA法检测血清抗 -HGV ,用HGVNS5抗原制备MCAb ,检测 5 4例慢性肝炎肝硬化患者肝组织中HGV抗原 ,同时应用SP法分别对肝组织进行HBsAg、HBcAg、HCVNS3 Ag肝脏免疫组化检测。结果提示 5 4例肝病患者肝组织检出 2 7 78%HGV抗原阳性。其中 2 4例慢性肝炎轻度中 5例 ;16例慢性肝炎中度中 5例 ;15例肝硬化中 5例。HGV重叠HBV或HCV二重感染及HBV、HCV三重感染为主 ,占 80 % ,单独HGV感染者占 2 0 %。HGV在肝脏内主要定位于胞浆型 ,部分同时核型表达或单独核型表达 ,有 1例单纯枯否氏细胞内HGAg阳性表达。HGV单独感染者临床隐匿性发病 ,预后好 ,HGV重叠HBV、HCV感染在慢性肝炎病期进展具有一定意义
To investigate the infection rate, clinical and pathological characteristics of HGV in chronic hepatitis and liver cirrhosis, serum anti-HGV was detected by ELISA, MCAb was detected by HGVNS5 antigen, and HGV antigen was detected in 54 liver cirrhosis patients with chronic hepatitis. The liver tissues were harvested for immunohistochemistry detection of HBsAg, HBcAg and HCVNS3 Ag, respectively. The results suggest that 57.4% of HGV antigens were positive in the liver of 54 patients with liver disease. Of these, 24 were moderately mild in 5, 5 were in 16 moderately chronic hepatitis, and 5 were in 15 with cirrhosis. HGV overlap HBV or HCV double infection and HBV, HCV triple infection, accounting for 80%, HGV infection alone 20%. HGV mainly located in the liver in the cytoplasm, part of karyotype expression or karyotype expression, there is a simple Kupffer cell HGAg positive expression. HGV infection alone clinically occult disease, the prognosis is good, HGV overlap HBV, HCV infection in the progress of chronic hepatitis stage has a certain significance