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作者发现发色底物ＢＯＣ－Ｌｅｕ－Ｓｅｒ－Ｔｈｒ－Ａｒｇ－ｐＮＡ对激肽释放酶具有良好的敏感性和特异性。它受激肽释放酶作用的Ｋｍ和Ｖｍａｘ。分别为２３５ｐｍｏｌ·Ｌ－１和３３７ｎｍｏｌ（ＰＮＡ）·Ｓ－１·Ｕ－１（ｋＫ）；抑制试验显示，该底物对ＡＴ－Ⅲ和ＬＢＴＩ不敏感，用作激肽释放酶测定的回收率为９７．２％～１０２．ｌ％，ＣＶ值２．３％～４．６％。用它建立了血浆激肽释放酶活性测定法，标准曲线线性甚佳，线性范围６．２５％～３００．０％（激肽释放酶活性）．测得健康人、妊娠妇女、肝功能衰竭病人和晚期癌症病人的血浆前激肽释放酶活性分别为１１３．９％，１４５．７％，４０．０％和９０．０％。作者认为，发色底物ＢＯＣ－Ｌｅｒ－Ｓｅｒ－Ｔｈｒ－Ａｒｇ－ｐＮＡ是ＰＫ（ＫＫ）活性测定的良好底物，建立的ＰＫ活性测定法简便、快速、敏感、可靠，实用价值大。
The authors found that the chromogenic substrate BOC-Leu-Ser-Thr-Arg-pNA has a good sensitivity and specificity for kallikrein. It is affected by the kallikrein Km and Vmax. 235 pmol·L-1 and 337 nmol (PNA) · S-1 · U-1 (kK) respectively. Inhibition assays showed that the substrate was insensitive to AT-III and LBTI and was used as a kallikrein assay for recovery 97.2% ~ 102. l%, CV 2.3% ~ 4.6%. Using it to establish a plasma kallikrein activity assay, the standard curve is very good, the linear range of 6.25% to 300.0% (kallikrein activity). Plasma kallikrein activities were measured in 113.9%, 145.7%, 40.0% and 90.0% of healthy subjects, pregnant women, patients with liver failure, and advanced cancers, respectively. The authors believe that the chromogenic substrate BOC-Ler-Ser-Thr-Arg-pNA is a good substrate for the determination of PK (KK) activity. The established PK activity assay is simple, rapid, sensitive and reliable and has great practical value.