Effect of flunarizine on HSP70 gene expression for following transient ischemia

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Objective To investigate the effects of flunarizine on HSP70 gene expression for following transient ischemia.Methods Northern blot and immunohistochmistry (IHC) were used to determine the expression of the HSP70 during different periods after post ischemic reperfusion with or without Flunarizine treatment in the gerbil. Results The HSP70mRNA expression was increased in the forebrain,but the HSP70 protein only expressed at the 1st day of reperfusion(P<0.05);Flunarizine treatment could increase the expression of HSP70 protein, but couldn’t significantly increase the expression of HSP70 mRNA (P >0.05). Conclusion Flunarizine could protect neurons from ischemic damage through increasing the HSP70 expression. Objective To investigate the effects of flunarizine on HSP70 gene expression for the following transient ischemia. Methods Northern blot and immunohistochistry (IHC) were used to determine the expression of the HSP70 during different periods after post ischemic reperfusion with or without Flunarizine treatment in the gerbil. Results The HSP70 mRNA expression was increased in the forebrain, but the HSP70 protein only expressed at the 1st day of reperfusion (P <0.05); Flunarizine treatment could increase the expression of HSP70 protein, but could not significantly increase the expression of HSP70 mRNA (P > 0.05). Conclusion Flunarizine could protect neurons from ischemic damage through increasing the HSP70 expression.
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