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目的:对8例ABO血型B亚型进行血清学检测并分析,为临床鉴定正反定型不符疑难血型提供借鉴。方法:采用微柱凝胶卡式法及试管法进行ABO血型定型,并应用红细胞吸收放散试验、唾液中HAB血型物质测定等鉴定亚型。结果:8例患者样本红细胞上均存在B抗原,并经血清学鉴定均符合Bx亚型分类标准。结论:Bx亚型由于红细胞所含B抗原极弱,易被误定为O型,在亚型红细胞出现正反定型不符的情况下,应使用包括血清学在内的多种方法正确鉴定其血型,以保证输血安全。
OBJECTIVE: To provide a reference for the analysis of 8 cases of ABO blood group B subtype by serological test and clinical analysis of positive and negative stereotypes. Methods: ABO blood type was determined by micro-column gel method and test tube method. The subtype was identified by erythrocyte absorption and desorption test and determination of HAB blood type in saliva. Results: B antigen was present on the erythrocytes in all 8 patients and was identified by serology as Bx subtype. CONCLUSION: Bx isoform is easily misidentified as O-type due to extremely weak antigen B contained in erythrocytes. In the case of positive and negative stereotyping of erythrocytes, the blood type should be correctly identified by various methods including serology , To ensure the safety of blood transfusion.